The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Volume 69, Issue 4 (15.12.2023)
| Volume : | 69 |
| Issue : | 4 (15.12.2023) |
| Authors : | Yuriy V. KARPETS, Dmyto A. TARABAN, Alexander I. KOKOREV, Tetiana O. YASTREB,Liubov N. KOBYZEVA, Yuriy E. KOLUPAEV |
| Title : | RESPONSE OF WHEAT SEEDLINGS WITH DIFFERENT DROUGHT TOLERANCE TO MELATONIN ACTION UNDER OSMOTIC STRESS |
| Abstract : | Melatonin is a multifunctional molecule whose biological activity has been intensively studied in recent years in the context of plant adaptation to abiotic stresses, including drought. However, comprehensive studies on the effect of melatonin on the status of antioxidant and osmoprotective systems in wheat varieties with different drought adaptation strategies have been lacking. We studied the effect of melatonin on the growth and functioning of stress protection systems of wheat (Triticum aestivum L.) seedlings of Tobak (drought-tolerant) and Doskonala (drought-sensitive) varieties. Two-day-old etiolated seedlings were transferred to a 15% PEG 6000 solution for 2 days to create a model drought. The addition of melatonin to the incubation medium of seedlings at concentrations in the range of 0.2-5.0 μM significantly alleviated the inhibition of root and shoot growth of seedlings caused by drought. The effects of melatonin were more pronounced in the drought-sensitive cultivar Doskonala. Melatonin treatment almost completely eliminated the effect of increased hydrogen peroxide content under drought conditions in both varieties. Also in Doskonala variety, melatonin treatment reduced stress-induced accumulation of lipid peroxidation products. Drought caused a decrease in superoxide dismutase activity in both varieties, while melatonin treatment helped to maintain the enzyme activity at a level close to that of the control variants. In both varieties, melatonin treatment also reversed the stress-induced decrease in guaiacol peroxidase activity. Under the influence of melatonin, proline accumulation was significantly enhanced in Doskonala under drought conditions. Drought caused a decrease in sugars and flavonoid compounds, while melatonin mitigated these effects in both varieties. It is concluded that melatonin significantly modulates the adaptive responses of etiolated wheat seedlings to drought and that its effects depend on cultivar characteristics. |
| For citation : | Karpets, Y.V., Taraban, D.A., Kokorev, A.I., Yastreb, T.O., Kobyzeva, L.N., Kolupaev, Y.E. (2023): Response of wheat seedlings with different drought tolerance to melatonin action under osmotic stress. Agriculture and Forestry, 69 (5): 53-69. doi:10.17707/AgricultForest.69.4.05 |
| Keywords : | Triticum aestivum, melatonin, drought tolerance, reactive oxygen species, antioxidant system, proline, soluble carbohydrates, flavonoids |
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