The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Volume 63, Issue 4 (15.12.2017)
| Volume : | 63 |
| Issue : | 4 (15.12.2017) |
| Authors : | Roksolana SOVINSKA, Ganna KOROTYEYEVA, Olena ANDRIYCHUK |
| Title : | PROSPECTIVE USE OF BACTERIOPHAGES AS AGENTS TO COMBAT PHYTOPATHOGENIC BACTERIA OF POTATOES |
| Abstract : | Plant diseases caused by bacteria are a serious problem in the cultivation and storage of agricultural products. During the last years bacteriophages have attracted increased research interest as a realistic environmentally friendly means for controlling bacterial diseases. The main objective of the study was to characterize the bacteriophages that could be used in the search for and developing the antimicrobial agents based on bacterial viruses. Four isolates of bacteriophages specific to bacteria from Pseudomonas genus were isolated from potato samples with typical symptoms of bacterial disease. These phage isolates differed in terms of the morphology of their negative colonies. Slight variation in the morphology of studied phages was observed using electron microscopy. A group of phages was identified as Podoviridae family of Caudovirales order (icosahedral head without long tail, small size – head diameter 43 ± 1 nm, tail length 1 ± 0,5 nm). To identify host range/specificity of isolated phages, we analyzed the spectrum of lytic activity against 15 strains of phytopathogenic bacteria. Our research revealed that among four phage samples three expressed lytic activity against different strains of phytopathogenic bacteria. The aforementioned data enable us to conclude that these three isolates with broad spectrum of lytic activity can be used as perspective biologic agents in control of bacteriosis. Thereby phages from our collection could be of therapeutic interest, they have the potential to be used in future prospects for phage therapy research. |
| For citation : | Sovinska R., Korotyeyeva G., Andriychuk O. (2017) Prospective use of bacteriophages as agents to combat phytopathogenic bacteria of potatoes. Agriculture and Forestry, 63 (4): 69-74. DOI:10.17707/AgricultForest.63.4.07 |
| Keywords : | bacteriophage, bacteriosis, biocontrol, potato |
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