Volume 60, Issue 1

Volume : 60
Issue : 1
Authors : Valbona SOTA, Efigjeni KONGJIKA
Abstract : This study is undertaken to develop a protocol for in vitro conservation of Zizyphus jujuba Mill. using different methods for mid-term storage. This specie is very important for the pharmacological values of fruits and leaves, and has a very common use in food industry and for its fragrance. For this reason, it would be of interest to use a method available for in vitro multiplication and medium-term germplasm conservation, which involves strategies to slow plant growth through chemical and environmental manipulation of in vitro conditions. Shoot tips, used as primary explants, were cultured onto MS medium supplemented with MS vitamins, BAP (6-benzilaminopurine) 1 mg l-1, IBA (3-indole bytiric acide) 0.05 mg l-1, 3% sucrose and 0,57% agar-agar. The pH value was established in 5.6. Three different methods of short and mid-term conservation were examined using in vitro grown plant cultures: 1- Effect of reduced sucrose and MS salts concentrations; 2- Combination of low temperature and light regime; 3- Absence of phytohormones or growth regulators in the growth media. Maintenance in these conditions reduced microcuttings growth. Were evaluated the survival and regeneration rate for different periods. To test the regeneration of the conserved cultures, they were transferred onto fresh culture medium. The examined methods differed significantly in the survival rate of the explants. The effect of low temperature (4oC) combined with reduced light regime is the most effective method of medium term preservation. The optimal time of conservation without subculture on 4oC was 10 months.
For citation : Valbona SOTA, Efigjeni KONGJIKA (2014): SLOW GROWTH IN VITRO CONSERVATION OF ZIZYPHUS JUJUBA MILL. Agriculture & Forestry, Vol. 60. Issue 1: 27-37, 2014, Podgorica
Keywords : In vitro conservation, survival, regeneration, Zizyphus jujuba
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ISSN 0554-5579 (Printed)
ISSN 1800-9492 (Online)
DOI : 10.17707/AgricultForest


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