Year 2022, Volume 68 Issue 3 (30.09.2022)

Year : 2022
Volume : 68
Issue : 3 (30.09.2022)
   
Authors : Anatoliy SOROKA, Viktor LYAKH
Title : HOW ENSURE IN VITRO POLLEN GERMINATION OF CULTIVATED FLAX AND ITS WILD RELATIVES
Abstract : Until recently, flax was considered a crop whose pollen does not germinate well under artificial conditions. At the same time, rapid acquisition of information on pollen quality is important in many studies. Two linseed varieties and its three wild relatives with n = 15 were used as pollen sources. The basic artificial nutrient medium for pollen germination consisted of 200 mg/L boric acid and 200 mg/L calcium chloride. Experimental treatments additionally included polyethylene glycol of various molecular weights and sucrose. The media with polyethylene glycol 2000 in concentrations of 20-30 % ensured good pollen germination of cultivated flax, showing about 40% of pollen grains with normal pollen tubes. For wild species pollen, 20 % osmotic concentration was more preferable. The addition of sucrose to a medium with polyethylene glycol 2000 disadvantageously influenced pollen germination of cultivated flax and did not affect this indicator for wild species. When polyethylene glycol of higher molecular weight was used in a nutrient medium as an osmotic, the germinated pollen grains usually had burst pollen tubes or did not germinate at all.
For citation : Soroka, A., Lyakh, V, (2022). How ensure in vitro pollen germination of cultivated flax and its wild relatives. Agriculture and Forestry, 68 (3): 285-292. doi:10.17707/AgricultForest.68.3.21
Keywords : cultivated flax, wild species, pollen, in vitro germination, osmotic agent
   
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