Year 2021, Volume 67, Issue 3 (30.09.2021)

Year : 2021
Volume : 67
Issue : 3 (30.09.2021)
Authors : Milan MARKOVIĆ, Dušica RADONJIĆ, Milena ĐOKIĆ, Aleksandra KANDIĆ, Božidarka MARKOVIĆ
Abstract : Genetic polymorphism of κ-casein gene was investigated in three cattle breeds reared in Montenegro: Brown Swiss, Busha and Gray breeds of cattle. The DNA of these breeds was genotyped for polymorphisms at the kappa-casein gene by a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) essay. A 351 bp fragment of κ-casein was amplified and digested with HinfI restriction endonuclease. Three genotypes were identified (AA, AB and BB) with frequencies of 0.162, 0.501 and 0.356 (respectively) in Brown Swiss breed, 0.267, 0.501 and 0.233 in Busha breed and 0.236, 0.500 and 0.264 in Gray cattle breed. The frequencies of alleles A and B were 0.403 and 0.597 (respectively) in Brown Swiss, 0.517 and 0.483 in Busha cattle and 0.486 and 0.514 in Gray cattle. The genotyping of κ-casein alleles (A and B) is of practical importance, since B allele is in positive correlations with commercially valuable parameters of cheese yielding efficiency. The results of high frequency of B allele in Brown Swiss cattle confirmed a good performance of this breed in Montenegrin population. Results could be used for possible increasing the frequency of desired alleles and genotypes by including it in the breeding program for Brown Swiss breed as well as in the preservation program for Gray cattle and Busha as the important animal genetic resources in Montenegro.
For citation : Marković, M., Radonjić, D., Đokić, M., Kandić, A., Marković, B. (2021): Allelic polymorphism of k-casein gene (csn3) in three Montenegrin cattle breeds. Agriculture and Forestry, 67 (3): 61-70
Keywords : κ-casein, PCR-RFLP, Busha, Brown Swiss, Gray cattle
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