Year 2020, Volume 66 Issue 1 (31.03.2020)

Year : 2020
Volume : 66
Issue : 1 (31.03.2020)
Authors : Oksana KUTSENKO, Irena BUDZANIVSKA
Abstract : The most sensitive viral disease of the stone crops is Sharka, the causative agent of which is the plum pox virus (PPV, Plum Pox virus). PPV worldwide has a quarantine status. PPV is widespread in many regions of Ukraine and poses a serious problem to horticulture stone crops of our country. The purpose of our research was to analyze the distribution and the harmfulness of this pathogen, to describe the ways of transmission, to carry out research on the identification of aphids that were carriers of the disease and to characterize its molecular features and strains diversity in the territory of Ukraine. The samples were visually selected from the central and northern regions of Ukraine. Modern methods of molecular diagnostics were used such as: polymerase chain reaction with reverse transcription, sequencing. The phylogenetic analysis confirmed the identity of the strains and helped us made a comparative characterization of the samples to the already known strains. Depending on the strain, different kinds and varieties of plants could be damaged and crop losses could significantly vary. Therefore, it is important to determine the diversity of PPV strains and their similarities with other isolates. The result revealed high level of damage to stone crops in the territory of Ukraine, especially in Odessa and the Kiev region. Harmfulness and distribution of this disease increases every year. These researches are needed to find ways to fight this pathogen and stop the spread of a dangerous virus in Ukraine.
For citation : Kutsenko, O., Budzanivska, I. (2020): Distribution and transmission of Plum pox virus in Ukraine. Agriculture and Forestry, 66 (1): 49-56
Keywords : Plum pox virus, distribution, strain diversity, PCR, phylogenetic analysis, Ukraine
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