The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2019, Volume 65 Issue 4 (15.12.2019)
| Year : | 2019 |
| Volume : | 65 |
| Issue : | 4 (15.12.2019) |
| Authors : | Khrystyna SHEREVERA, Tetiana SHEVCHENKO, Halina SNIHUR, Irena BUDZANIVSKA, Oleksiy SHEVCHENKO |
| Title : | FIRST REPORT OF LEEK YELLOW STRIPE VIRUS ON ALLIUM SATIVUM L. IN UKRAINE |
| Abstract : | Garlic (Allium sativum L.) is a valuable crop, cultivated all over the world. Viral diseases of garlic are one of the factors that reduce quality of garlic yield. Leek yellow stripe virus belongs to genus Potyvirus, family Potyviridae, and garlic infected with LYSV is found in many places of garlic production. In 2018, we screened garlic-growing areas in different regions of Ukraine and collected plants with symptoms characteristic of viral infection: yellow striping and streaking, mosaic, spotting and plant growth retardation. DAS-ELISA with commercial diagnostic kit for LYSV (Adgia, USA) showed that 28% of collected plants were infected with LYSV. LYSV was detected in Vinnytsia region (Bershad district), Kyiv region (Boryspil district), and Poltava region (Semenivka district) in Ukraine, suggesting wide spread of the virus. According to available literature data, LYSV is often found in coinfection with OYDV. However, we found only 7% of plants to be mixed infected (LYSV+OYDV). Subsequent transmission electron microscopy revealed viral particles of 720-800 nm in length and 16 nm in diameter as characteristic for Potyvirus representatives. Further, LYSV infection was also confirmed by RT-PCR with coat protein gene-specific primers generating LYSV cDNA of expected length (~409 bp). This is the first report of LYSV-infected garlic plants in Ukraine that proves LYSV is widely spread in Ukraine |
| For citation : | Sherevera, K., Shevchenko, T., Snihur, H., Budzanivska, I., Shevchenko, O. (2019): First report of leek Yellow Stripe Virus on Allium sativum L. in Ukraine. Agriculture and Forestry, 65 (4): 47-53. DOI:10.17707/AgricultForest.65.4.05 |
| Keywords : | leek yellow stripe virus, onion yellow dwarf virus, garlic, potyvirus, Ukraine |
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