Year 2018, Volume 64 Issue 1 (31.03.2018)

Year : 2018
Volume : 64
Issue : 1 (31.03.2018)
Authors : Natalia YEGOROVA, Valentina BRAILKO, Irina STAVTZEVA, Irina MITROFANOVA
Abstract : The data about some morpho-physiological peculiarities of explants of lavender (Lavandula angustifolia Mill.) the cultivars Stepnaya, Sineva, Vdala, Record, Rannyaya, introduced by meristems in aseptic conditions and under long-term micropropagation in vitro are presented. During cultured meristems on Murashige and Skoog (MS) medium with 1.0 mg L-1 Kinetin and 0.5 mg L-1 GA3 all cultivars have formed the multiple shoot. The maximum number of microshoots at in vitro introduction stage was in the cultivar Sineva (5.7), and minimum – in the cultivar Rannyaya (3.9). Further, microcutting of main and additional shoots was conducted every 30-35 days. Obtained shoot segments with one node on MS medium with 1.0 mg L-1 Kinetin and 0.5 mg L-1 GA3 were cultured. When micropropagation of lavender follow 6th subculture it was shown that the number of shoots was increased in all cultivars till 3 subculture, and then it was decreased. In the cultivar Sineva the maximal multiplication index (12.2) was noted at the third passage, and then changed from 11.5 to 7.4. The lowest multiplication index at 5-7th passages was in the cultivar Record (3.8-4.5). Analysis of water regime of obtained microshoots at 6th subculture showed that the total water content was 83-89%, and the fraction of bound and colloid-absorbed water was 40-57%. Maximum values of water-holding capacity in the cultivars Record, Rannyaya and Vdala were found. Studying the parameters of light-induction processes it has been showed that the cultivars Vdala and Sineva were characterized by maximum photosynthetic activity of leaves and shoots, and they had high viability index.
For citation : Yegorova, N., Brailko, V., Stavtzeva, I., Mitrofanova, I. (2018): Some morphophysiological features of lavander cultivar micropropagated in vitro by meristem culture. Agriculture and Forestry, 64 (1): 105-111. DOI:10.17707/AgricultForest.64.1.13
Keywords : Lavandula angustifolia, clonal micropropagation, multiplication index, water regime, photosynthetic activity
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