Year 2012, Volume 58 Issue 3

Year : 2012
Volume : 58
Issue : 3
   
Authors : Doriana BODE, Efigjeni KONGJIKA, Skerdilaid XHULAJ
Title : USE OF BIOTECHNOLOGIES METHODS IN MULTIPLICATION OF NATIVE PRUNUS SP. GERMPLASM
Abstract : The present study was carried out to investigate the use of “in vitro” methods as an alternative form of propagation for two different Prunus germplasm native resources. Shoot tips explants collected in the Tirana region were cultured in an MS medium supplemented with different types of plant growth regulators (ANA, BAP and GA3) during the proliferation and multiplication phases. Results were elaborated with factorial experiment with two factors in completely random design layout. During proliferation, apricot explants were recorded as having higher values for the shoot length parameter (±10.6mm) in comparison with that of plum explants (± 6.3mm). Different results were observed for leaf parameters, where plum shoot tips presented a higher number of leaves (±8.6) than apricots (±5.2). During subculture, medium composition positively affects the new plantlets biometry, giving plum plantlets the highest number of leaves (±16) and new apricot plantlets the highest value of shoot length (±2.2cm). The multiplication coefficient recorded for the two Prunus plantlets was more than 4 buds/explants. Our results suggested that the method used in this study was appropriate for the multiplication of the studied Prunus germpalsm.
For citation : Doriana BODE, Efigjeni KONGJIKA, Skerdilaid XHULAJ (2012): USE OF BIOTECHNOLOGIES METHODS IN MULTIPLICATION OF NATIVE PRUNUS SP. GERMPLASM. Agriculture and Forestry, Vol. 58. Issue 3: 29-38, 2012, Podgorica. ISSN 0554-5579 (Printed) ISSN 1800-9492 (Online).
Keywords : propagation, Prunus, germplasm, growth regulators, medium
   
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